Validation of the direct polymerase chain reaction tests performed by the National Public Health Laboratory does not give accurate results in all samples of the infected people tested in the laboratory.

Direct polymerase chain reaction test is a method of DNA amplification directly, without performing DNA isolation and purification steps. Doctors say the technique greatly reduces experimental time and costs.

“The results of the validation tests we performed gave only satisfactory results and results of tests carried out in other laboratories were even worse,” Dr Runa Jha, director at the National Public Health Laboratory, told the Post. “Results started to fluctuate in the samples having cycle threshold (CT) value above 31.”

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